Enhanced analytical sensitivity of a quantitative PCR for CMV using a modified nucleic-acid extraction procedure
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چکیده
منابع مشابه
Enhanced analytical sensitivity of a quantitative PCR for CMV using a modified nucleic-acid extraction procedure.
Accurate and rapid diagnosis of CMV disease in immunocompromised individuals remains a challenge. Quantitative polymerase chain reaction (QPCR) methods for detection of CMV in peripheral blood mononuclear cells (PBMC) have improved the positive and negative predictive value of PCR for diagnosis of CMV disease. However, detection of CMV in plasma has demonstrated a lower negative predictive valu...
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We examined the effect of nucleic acid extraction methods on the analytic characteristics of a quantitative polymerase chain reaction (PCR) assay for cytomegalovirus (CMV). Human serum samples were extracted with 2 automated instruments (BioRobot EZ1 and QIAsymphony SP, QIAGEN, Valencia, CA) and CMV PCR results compared with those of pp65 antigenemia testing. Both extraction methods yielded res...
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Polymerase chain reaction (PCR) has been widely used due to its high specificity, sensitivity, and rapid turn-around time. However, the major limitation of PCR based diagnosis is inhibition of the reaction caused by a variety of components within clinical specimens. The demand for PCR diagnosis in medical microbiology has highlighted the need for efficient methods of nucleic acid extraction. Al...
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ژورنال
عنوان ژورنال: Journal of Clinical Laboratory Analysis
سال: 2000
ISSN: 0887-8013,1098-2825
DOI: 10.1002/(sici)1098-2825(2000)14:1<32::aid-jcla7>3.3.co;2-n